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Advanced HPLC Method Development Using Quality by Design


An intensive online course that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence.


What Does it Cover

“Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

The course covers:

  • What Quality by Design (QbD) is and how it applies to HPLC methods
  • A review of basic HPLC theory as it applies to method development
  • The makeup of HPLC columns including the support material and the bonded phase
  • The role of solvents in the separation
  • How the column plate number, selectivity, and retention can be used in a logical way to solve even the toughest separation problems.
  • How to use mobile phase solvents, pH, temperature, chemistry, and other variables to pull apart difficult-to-separate peak pairs.
  • How gradient elution works and how to remove the mystery of optimizing gradients.
  • How a scouting gradient can be used to speed the development of both isocratic and gradient methods.
  • How to use a simple spreadsheet calculator to move from the scouting run to the next step in method development
  • How computer-assisted method development tools can leverage a small amount of data into a large amount of information about a separation
  • How to take advantage of a publicly available internet database to identify equivalent and orthogonal columns
  • When standard reversed-phase techniques don’t work, how to choose mixed-mode, ion-pairing, normal phase, HILIC, chiral, or other chromatographic modes
  • What’s the same and different when comparing HPLC and UHPLC
  • What to look for when validating a method

What Will You Learn

You should gain a better understanding of the chromatographic process and how it applies to HPLC and UHPLC method development. You will learn practical “tricks of the trade” from experts in developing methods in the chemical, pharmaceutical, and bioanalytical laboratory. You will obtain a step-by-step procedure to apply to the development of reversed-phase HPLC and UHPLC methods for nearly any sample. You will get copies of all the slides shown in the class to use as a resource after the class. You will find that a systematic approach to method development has a great advantage over trial-and-error or modifying an older method, in terms of speed of development, faster methods, and more robust separations.

Who Should Take This Course

This course is designed for practical workers in the laboratory who have responsibility for designing new HPLC or UHPLC methods or transferring methods between laboratories. Experienced workers will get the most out of this class, but because it builds from the fundamentals, anyone with experience in HPLC or UHPLC will gain valuable knowledge.



Additional Information



For more information on the learning materials included in this courses click on the link below...


Register for Free Access

Access all HPLC, GC & CE Courses

Duration: 6 months (fixed length)

Separation Science is pleased to offer free access to all HPLC and GC courses - this includes:

  • Fundamentals of HPLC
  • HPLC Basics, Equipment & Troubleshooting
  • HPLC & UHPLC Troubleshooting
  • Advanced HPLC Method Development
  • Principles of HPLC Validation
  • Practical HPLC for Biopharmaceuticals
  • LC-MS/MS for Chromatographers
  • Introduction to GC
  • Intermediate GC
  • GC-MS for Operators & Method Developers

All you need to do is register...

Please direct all questions on Analytical Training Solutions to Jackie Tan