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LC-MS/MS for Chromatographers


An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices


What does it cover

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquidchromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs inbiological matrices in the pharmaceutical laboratory. As they have become more widely used and understood,LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis,and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC andMS to a routine tool usable much as any other HPLC detector.

You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding thetechnique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ionsare produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

What Will You Learn

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise. You will receive a printed copy of the approximately 515 slides included in this course to supplement your note-taking as you view the class.

Who Should Take This Course

This course is designed to provide basic knowledge and practical applications of LC-MS for liquid chromatographers. Most of the examples emphasize the most popular use of LC-MS/MS today: the analysis of drugs in metabolites in biological extracts. Although no previous experience with HPLC is necessary, the course will be especially valuable to those with some chromatography experience.



Additional Information



For more information on the learning materials included in this courses click on the link below...


Register for Free Access

Access all HPLC, GC & CE Courses

Duration: 6 months (fixed length)

Separation Science is pleased to offer free access to all HPLC and GC courses - this includes:

  • Fundamentals of HPLC
  • HPLC Basics, Equipment & Troubleshooting
  • HPLC & UHPLC Troubleshooting
  • Advanced HPLC Method Development
  • Principles of HPLC Validation
  • Practical HPLC for Biopharmaceuticals
  • LC-MS/MS for Chromatographers
  • Introduction to GC
  • Intermediate GC
  • GC-MS for Operators & Method Developers

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Please direct all questions on Analytical Training Solutions to Jackie Tan